Fermentation of Glucose by Fusarium Lini 



109 



flasks were sterilized and inoculated with a definite volume of a 

 spore suspension of Fusarium lini and incubated at 28 to 30 

 C.°. In the experiment here reported 15 flasks were used, the P H 

 being adjusted to 5.545, which is within the optimum reaction for 

 this organism. These flasks were divided into sets of three each, 

 and at intervals sets were removed and analyzed. Flask 1 was 

 used for determining alcohol ; flask 2 for mycelium, glucose, and 

 lead precipitate; and flask 3 for mycelium and P H . The C0 2 was 

 determined on each flask at frequent intervals to avoid the de- 

 velopment of pressure. The mycelium was separated from the 

 medium by filtration onto a gooch. The carbon in the original 

 culture, in the mycelium, and in the lead precipitate, was deter- 

 mined by wet combustion; alcohol was determined by aeration 

 into concentrated H 2 S0 4 , oxidation with potassium dichromate 

 to acetic acid, and distillation and titration of the latter; and 

 glucose by the picramic acid method. Since in a previous experi- 

 ment succinic acid had been identified as a product of the action 

 of the organism on glucose, it was thought that lead acetate would 

 precipitate any of this, hence the determination of the lead pre- 

 cipitate. 



Chart I shows the results of the experiment. It is evident that 

 over 90 per cent, of the glucose can be accounted for and that 



