On the Estimation of Organic Phosphorus 171 



pletely sterilized at the end of five hours. Albolene after an 

 initial drop at the end of one hour, showed a marked increase 

 in the number of organisms after that time. Cottonseed oil and 

 peach kernel oil showed an increase of organisms at the end of 

 the first hour. Oil of almonds although greatly reducing the 

 number of organisms in five hours showed a marked increase in 

 organisms before that time. The most striking observation in 

 this study is the antiseptic effect of olive oil. This could not 

 be attributed to impurity or acidity of the oil as only the purest 

 product, neutral in reaction, was employed in the tests. The 

 above findings may be of interest to such clinicians as larynolo- 

 gists, rhinologists and others who have occasion to use solutions 

 of antiseptics and other drugs in oil. 



ACTION OF OILS AGAINST STAPHYLOCOCCUS PYOGENES AUREUS 



Oil. 



1 minute. 



1 hour. 



3 hours. 



5 hours. 





Number of 

 organisms 

 per c.c. 



Per cent. 



Number of 

 organisms 

 per c.c. 



Per cent. 



Number of 

 organisms 

 per c.c. 



Per cent. 



Number of 

 organisms 

 per c.c. 



Per cent. 



Olive oil 



110,000 

 136,000 

 59,000 

 1,400,000 

 530,000 



100 

 100 

 100 



100 • 

 100 



16,50C 

 145,000 

 1,500 

 5,150,000 

 1,100,000 



15. 

 106.6 



25.4 

 367.8 

 218.8 



1,350 

 30,910 

 67,500 

 250,000 

 1,885,000 



1.2 

 22.7 



144.4 

 17.8 



355.6 



0 



25,450 

 67,500 

 16,000 

 440,000 



0. 

 18.7 

 144.4 

 1.1 

 83.0 



Cotton seed oil... . 

 Albolene 



Oil of almonds... 

 Peach kernel oil.. 



79 (2039) 



On the estimation of organic phosphorus. 

 By EMIL J. BAUMANN. 



[From the Laboratory Division, Montefiore Hospital, New York 



City.] 



In attempting to use the methods of Bloor and Bell and Doisy 

 for estimating liopoid phosphorus, difficulty was experienced in 

 securing uniform results. A study of these methods was there- 

 fore made using pure inorganic phosphate solutions. Complete 

 recovery was rarely possible and the apparent losses were quite 

 variable. After excluding other steps in the processes as possible 



