178 



Scientific Proceedings (127) 



83 (2043) 



The presence and determination of adenine nucleotide in human 



blood. 



By HENRY JACKSON, JR. 



[From the Department of Medicine of the College of Physicians 

 and Surgeons, Columbia University, and the Presbyterian 

 Hospital, New York.] 



Tungstic acid protein free blood filtrate was hydrolyzed with 

 dilute mineral acid for two hours, then made strongly alkaline 

 with ammonia and evaporated slowly to a small volume. After 

 filtration, the filtrate was made alkaline with ammonia and pre- 

 cipitated with silver chloride in ammonia. The washed precipi- 

 tate was decomposed and picric acid added. The resulting crys- 

 taline precipitate was purified and one analysis proved to have 

 29.3 per cent, nitrogen. It melted at 281 C. After removal of 

 the picric acid, the residue proved to have approximately 38 per 

 cent, nitrogen. It precipitated with gold chloride and ammon- 

 iacal silver. It gave no color with Nessler's solution and no 

 blue color with alkaline phosphotungstate. 



Tungstic acid filtrate was precipitated with silver, the precipi- 

 tate broken up and the filtrate precipitated with neutral lead 

 acetate in acid solution. The lead precipitate was decomposed 

 and then gave a strong pentrose reaction with orcin and an ab- 

 sorption band between the D and the E. With napthoresorcin 

 the color produced did not shake out with ether or benzol. 

 There was no xanthin test nor was there any trace of blue with 

 phosphotungstate. 



If the blood filtrate was first hydrolyzed with acid and then 

 the same procedures carried out no traces of pentrose reaction 

 could be found. 



If the blood filtrate was precipitated with silver, then lead, 

 and the filtrat from lead sulphide was hydrolyzed with acid 

 subsequent addition of silver precipitated the nitrogen contain- 

 ing portion of the substance, while the substance producing the 

 pentrose reaction remained in the filtrate. 



If the inorganic phosphates are removed from the filtrate 

 from lead sulphide there is no immediate test for phosphates 



