Study of the Form and Growth of a Diphtheroid 179 



with ammonium molybdate. If, however, this same filtrate, 

 free from inorganic phosphates be hydrolyzed with dilute acid 

 before the test is done a yellow precipitate characteristic of 

 phosphates appears immediately. 



To tungstic acid blood filtrate was added under definite con- 

 ditions uranium acetate. The washed precipitate was decom- 

 posed and the resulting filtrate on digestion gave a nitrogen 

 content of about 4 mgm. of nitrogen per 100 c.c. whole blood. 

 Blanks were negative. Adenine nucleotide added to blood was 

 recovered quantitatively by this method. 



Conclusions. Adenine, probably bound is present in consid- 

 erable quantities in normal human blood. There is some evi- 

 dence presented to show that it is bound in the form of adenine 

 nucleotide. 



A method is outlined whereby nucleotides may be determined 

 quantitatively in small samples of tungstic acid blood filtrate. 



It is suggested that a large part of the undetermined nitrogen 

 in the tungstic acid filtrate of Folin and Wu is adenine nucleo- 

 tide. 



The work is being continued at The Thorndike Memorial 

 Laboratory of the Boston City Hospital. 



Abstracts of Communications, Minnesota Branch 

 Eighth meeting. 

 Minneapolis, Minnesota, December 13, 1922 



84 (2044) 



A statistical study of the form and growth of a diphtheroid 



bacillus. 



By ARTHUR T. HENRICI. 



[From the Department of Bacteriology and Immunology, Univer- 

 sity of Minnesota, Minneapolis, Minn.] 



In a previous communication 1 I described changes in the size 

 of the cells of Bacillus megatherium during the growth of a 



i-Proc. Soc. Exp. Biol, and Med., 1921, xix, 132. 



