190 



Scientific Proceedings (127) 



were small. The diet used should not have caused any excretion 

 of acetone if Shaffer's formula 2 is applied; the combustion of 

 varying mixtures of ketogenic and antiketogenic compounds in 

 different parts of the organism and at different times during 

 the day will probably give rise to such amounts of acetone as 

 these when the total amount of antiketogenic material is some- 

 what in excess of the ketogenic. 



After six days on the diet described the fat was discontinued 

 for three days, and the same amounts of protein and carbohy- 

 drate were fed as before. During this time body fat was pre- 

 sumably burned to replace the fat omitted from the diet. The 

 diet fed at the beginning of the experiment was then resumed 

 for a period of two days. There were no differences in the 

 amounts of acetoaecetic acid, /?-hydroxybutyric acid, and nitrogen 

 excreted in the three periods. Samples of blood taken before 

 breakfast before the experiment began, after six days on the 

 high fat diet, and after three days on the low fat diet contained 

 the same amounts of cholesterol (determined by the method of 

 Bloor 3 and of fat (determined by the methods of Bloor 4 and 

 of Gage 5 . 



From these results it was concluded that body fat and in- 

 gested fat (at least when the latter is not present in very great 

 amounts) gives rise to the same amounts of the acetone bodies 

 when metabolism takes place under comparable conditions. 



2 J. Biol Chem., 1922, liv, 399. 



3 J. Biol Chem., 1916, xxiv, 227. 



4 J. Biol Chem., 1917, xxxi, 375. 



5 Cornell Veterinarian, 1920, x, 154. 



