198 



Scientific Proceedings (127) 



0.3 gram of thyroid extract every second day, the activity was 

 maintained above that of the normal sheep but no sudden and 

 pronounced increase was observed. 



97 (2057) 



Influence on the respiratory metabolism of pancreatic extract 

 administered by mouth to der ancreatized dogs. 



By C. B. F. GIBBS and JOHN R. MURLIN. 



[From the Physiological Laboratory of the University of Roches- 

 ter, Rochester, N. Y.] 



Extracts of beef pancreas prepared in the same manner 1 as 

 extracts of dog's pancreas were prepared by Murlin and Kramer 2 

 in 1913-6 and administered by mouth to depancreatized dogs 

 with glucose and NaOH in sufficient quantity to make the en- 

 tire mixture N/20 NaOH produced in all of four trials distinct 

 elevations of the respiratory quotients taken from two to four 

 hours afterward. In two of the four trials the extract was con- 

 centrated and purified before administration and in both in- 

 stances the rise in quotient was greater than when crude un- 

 concentrated extract was given. 



One of the experiments on a depancreatized dog is repro- 

 duced below. 



Dog No. 40. Operated Nov. 28th, 1922. 



Date. 



Time. 



CO 



2 





O 



2 





BQ 



Dec. 1 



12:40-1:35 



2.943 





4.271 





0.689 





1:35-2:47 



4.073 





6.151 





0.662 





2:47-3:48 



3.330 





4.799 





0.694 



Dec. 2 



11:05-11:43 



1.960 





2.596 





0.755 





11:43-12:13 



1.578 





1.959 





0.721 





12:13-12:46 



1.619 





2.508 









3:40 P.M. Dog given 50 c.c cone. 



No. 87(2) 



extract in 







300 c.c water 



+ 20 



gm. glucose 



+ 



N/20 







NaOH (final 



reaction). 









4:53-5:23 



1.497 





2.854 





0.757 





5:23-6:23 



2.932 





3.813 





0.769 





6:23-7:41 



3.966 





4.479 





0.885 





7:41-8:42 



3.284 





3.424 





0.959 



' Murlin, 



Cramer and Sweet, 



Joum. Metabolic Besearoh, 1922, ii 



j 19. 





2 Murlin 



and Kramer, Joum. 



of Biol. Chevn. 



, 1916, 



xxvii, 516. 







