358 Scientific Proceedings (131) 



loops toxic compounds closely resembling primary proteoses in 

 all their properties. Cooke, Rodenbaugh and Whipple 4 have 

 shown that in cases of acute intestinal obstruction, whether pro- 

 duced experimentally in animals or occurring in human cases, 

 is accompanied by a rapid rise in the non-protein nitrogen of 

 the blood, from 3 to 10 times normal figure. However, in 

 chronic cases there may be little or no increase in the blood non- 

 protein nitrogen. These intoxications also show an increased 

 blood content of urea and creatinine. The authors state that the 

 kidneys in all of these cases are practically normal (anatomically) 

 and that the protein or tissue destruction, rather than impaired 

 renal function, is responsible for the rise in the blood nitrogen. 

 Rabinowitch 5 reports a significant increase in the urea nitrogen 

 of the blood in patients with intestinal obstruction. In many 

 cases it rose to more than 100 mg. per 100 c.c With this rise 

 in urea nitrogen the author noted a normal phenolsulphoneph- 

 thalein excretion by the kidneys, and he ascribed the increased 

 urea nitrogen to tissue destruction. Haden and Orr 6 noted after 

 ligation of the duodenum, ligation of the duodenum with gas- 

 troenterostomy and a ligation of the upper half of the ileum in 

 dogs, a rise in the non-protein and urea nitrogen and C0 2 com- 

 bining power of the blood, but a fall in the chlorides. The uric 

 acid, creatinine, amino acid nitrogen and sugar remained un- 

 changed. Ligation of the ileum at the ileocecal valve is followed 

 by but a slight increase in the nitrogen, and no change in the 

 C0 2 combining power. These authors believe that the blood 

 urea nitrogen is a good index of the protein destruction. 



At present report we are including only data on non-protein 

 nitrogenous compounds of the blood, the sugar and C0 2 c.p. 

 Hiller and Van Slyke 7 in a recent paper have shown after a com- 

 prehensive study of effect of the various protein precipitants 

 used in blood analysis upon the constituents of the non-protein 

 nitrogen, that precipitation of the proteins with 2.5 per cent, 

 trichloracetic acid is the preferable method in analyses concerned 

 with the undetermined nitrogen. Our procedure has been to 



4 Cooke, J. V., Rodenbaugh, F. H., and Whipple, G. H., Expcr. Med., 

 1916, xxiii, 717. 



• r > Rabinowitch, I. M., Canad. Med. Assoc. J., 1921, xi, 163. 



« Haden, R. L., and Orr, T. G., J. Exper. Med., 1923, xxxvii, 365 and 377. 



7 Hiller, A., and Van Slyke, D. P. ; Jour. Biol. Chcm, 1922, lii, 253. 



