Hydrogen Ion in the Blood 



365 



harmonizing with the C0 2 combining power in both instances. 

 In the first of these cases there was a rise to P H 7.33 four hours 

 after administration of insulin. In case 6 suffering from uremia 

 following a bladder operation, and dying several hours after 

 the test, the P H was 7.15 and the C0 2 20. In case 7 suffering 

 from advanced nephritis with marked nitrogen retention (blood 

 creatinine of 9 mg.), the P H was 7.30 and the C0 2 content 47. 

 In the case with cardiac decompensation there appears to be a 

 slight reduction in the P H . Case 9, following a cholecystectomy 

 operation and alkali therapy, showed a C0 2 combining power 

 of 98. Six hours later when a P H of 7.53 was observed the 

 C0 2 had fallen to 87. On the next morning the P H was 7.46 

 and the C0 2 was 79. 



176 (2136) 



Some studies on the vital staining of blood cells. 

 By R. SPIRIDONOVITCH (by invitation). 



[From the Department of Anatomy, Cornell University Medical 

 College, New York City.] 



There are many papers on the vital staining of the blood 

 cells but, considering the many papers of the conventional method 

 used in the examination of the blood, one may say that the field 

 of the vital staining is rather unexplored. It is surprising to 

 find how few have actually studied living cells. Most of the 

 work on vital staining is in reality supra vital, that is to say 

 the living cells on taking up the stain died. I will mention of 

 many investigators the names of Rosin and Biebergeil, Sabin 

 and E. Cowdry. These writers observed cytoplasmic granules 

 which took up certain dyes, while the cells containing them con- 

 tinued to live. 



I have made a few comparative studies of the effect of cer- 

 tain vital dyes on the cytoplasmic granules in the white blood 

 cell of man. To introduce the dye into a drop of blood diluted 

 with Ringer's solution, I used the following method, which was 



