Intranuclear Inclusion Bodies 



401 



ulation. The procedure adopted was as follows : A virus ob- 

 tained from a herpetic vesicle of the human lip was inoculated 

 upon the scarified cornea of a rabbit and was transferred from 

 cornea to cornea at two- or three-day intervals. The intranu- 

 clear bodies were found characteristically in corneas thus inocu- 

 lated. Purulent secretion was collected twenty-four hours after 

 inoculation of a cornea, suspended in saline solution and in- 

 jected in small quantities with a hypodermic syringe into rab- 

 bits as follows : directly into the testicle, into the brain after 

 trephining the skull, and into the abdominal organs after 

 laparotomy. The tracheal mucosa, the abdominal skin and the 

 muco-cutaneous border of the lip were scarified and purulent 

 conjunctival secretion was rubbed into the scarifications. The 

 inoculated areas were excised twenty-four hours after inocula- 

 tion, fixed in Zenker's solution and stained with haematoxylin- 

 eosin and with methylene-blue-eosin. 



Characteristic intranuclear bodies like those described by Lip- 

 schiitz have been found in the brain, trachea, testicle, adrenal' 

 liver, muco-cutaneous border of the lip and skin of the abdomen. 

 The bodies were observed in both nerve cells and glial cells of 

 the brain, in the ciliated epithelium of the trachea, in the intersti- 

 tial cells of Leydig of the testicle, in the cortical cells of the 

 adrenal, in the parenchymatous cells of the liver and the 

 squamous epithelial cells of the skin. Only within cells within 

 the lesion could these bodies be demonstrated. Herpetic virus 

 was shown to be present in the inoculated brain, testicle and 

 adrenal by inoculation of the rabbit's cornea. 



We believe that demontstration of these characteristic intra- 

 nuclear bodies within the nuclei of cells in the inoculated area 

 is diagnostic of a take* indicating proliferation of the virus of 

 herpes labialis locally. 



