Ethoxyquinolin and Chitenin 405 



more sensitive to the basic dyes than to the acid dyes, but the 

 Gram negative bacili are more sensitive to the acid dyes. 



In order to study this questions further we have tested the anti- 

 septic action of H acid (aminonaphthol disulphonic acid), 



OH NH 2 

 /\ /\ 

 / \/ \ 



I A J 



HOS0 2 \/ \/ S0 2 OH 

 an acid which is present in both trypan blue and the new 

 trypanosome remedy (Baeyer 205 7j )* The results are shown 

 in the table above. 



It is evident that the Gram positive cocci tested here are more 

 sensitive to H acid than are the Gram negative bacilli, and there- 



fore that Churchman's findings cannot be regarded as evidence 

 of an entirely general rule. 



197 (2157) 



The synthesis and excretion of hippuric acid: The glycine factor. 



By F. B. KINGSBURY. 



[From the Biochemical Laboratory of the Department of Phy- 

 siology, University of Minnesota.] 



The rate of synthesis and excretion of hippuric acid in normal 

 individuals and those with various renal disorder has already 

 been studied by Kingsbury and Swanson 1 and this study made 

 the basis of renal function test. In this work 2.4 grams of so- 

 dium benzoate was the dosage regardless of the size or weight 

 of the individual. In the present paper the original work has 



4 Churchman, J., Jour. Exper. Med., 1913, xvii, 373; Proc. Soc. Exper. 

 Biol, and Med., 1922, xix, 288-31. 



* A sample of pure H acid was kindly prepared for us by Drs. Derick 

 and Strachan, of the laboratories of the National Aniline Company, and the 

 bacterial cultures were furnished by Dr. J. F. Noble, to whom we extend 

 our sincere thanks. 



5 Bayer 205, Science, 1922, lvi, 514. 



i Kingsbury, F. B., and Swanson, W. W., J. Biol. Chem., 1921, xlv, 4; 

 Arch. Int. Med., 1921, xxviii, 220. 



