Liver Metabolism 



409 



Abstracts of Communications. 



Western New York Branch. 

 Sixth meeting. 



Rochester, New York, April 14, 1923. 



198 (2158) 

 A method for the study of liver metabolism. 

 By KARL F. CORI and G. T. CORI (by invitation). 



[From the State Institute for the Study of Malignant Disease, 



Buffalo, N. Y.] 



A method has been described which allows one to obtain 8-10 

 pieces of liver of the size of one gram under entirely physiolog- 

 ical conditions. A special apparatus has been constructed' con- 

 sisting of one part which is sewn in into an opening of the peri- 

 toneal cavity just below the sternum and of a cover which can 

 be screwed off. Three days after the operation the animal can 

 be considered as normal. It has a normal food intake. No dis- 

 turbances in the motility of the bowels have been observed. By 

 screwing off the cover of the window nearly every lobe of the 

 liver can be reached. Bleeding is entirely avoided by using 

 slightly heated scissors or Squibbs thromboplastin. The animal 

 shows no signs of excitement or pain. No increased adrenaline 

 production with following hyperglycemia occurs. If all opera- 

 tions are performed aseptically one can remove the window and 

 the animal will survive. A further advantage of this method is 

 that solutions can be injected directly into the stomach. 



Five lantern slides were shown, illustrating the usefulness of 

 this method. In two experiments the free sugar in the liver 

 was determined simultaneously with the bloodsugar after the 

 administration of adrenaline. One experiment was given show- 

 ing the effect of ingestion of 5 grams of glucose on the free 

 sugar and glycogen in the liver and the bloodsugar. Two similar 

 experiments were shown when glucose and iletin were given at 

 the same time. 



