Chylomicrons 



463 



amount of blood it is obvious that it might be of distinct ad- 

 vantage in clinical work. 



With this point in view several experiments were carried out 

 determining the number of chylomicrons in the blood by the 

 Gage and Fish method 2 and the total cholesterol and total fatty 

 acids by the method of Bloor, Pelkan and Allen. 1 



The experiments were as follows : 



Experiment 1 — Dog 14 was a healthy female mongrel weigh- 

 ing 7.75 kg. A specimen of blood was taken and the dog then 

 fed by mouth 70 c.c. of olive oil. Specimens of blood were then 

 taken two hours after feeding and every two hours thereafter 

 up to eight hours. 



Experiment 2 — Dog 15 was a healthy male weighing 17 kg. 

 A specimen of blood was taken and then the dog was fed by 

 mouth 70 c.c. of olive oil. Specimens of blood were taken as 

 in Experiment 1. 



Experiment 3 — Dog 14 as above was given 25 c.c. of glycerol 

 mixed with about 100 c.c. of water and specimens of blood taken 

 before feeding and every two hours thereafter. 



TABLE I. 







Whole Blood. 





Number 



















of 



Time. 



Total . 



Total Fatty 



Number of 



Experiment. 





Cholesterol. 



Acids. 



Chylomicrons. 



1 





mg. per 



mg. per 









100 c.c. 



100 c.c. 









180 



261 



19 





Before 





2 hrs. 



178 



290 



180 





4 hrs. 



172 



314 



150 





6 hrs. 



173 



322 



186 





8 hrs. 



173 



471 



156 



2 



Before 



171 



281 



8 





2 hrs. 



172 



323 



145 





4 hrs. 



180 



299 



121 





6 hrs. 



184 



307 



99 





8 hrs. 



180 



423 



170 



3 



Before 



191 



352 



37 





2 hrs. 



194 



355 



40 





4 hrs. 



196 



345 



62 





6 hrs. 



191 



353 



100 





8 hrs. 



182 



348 



89 



4 



Before 



68 



156 



51 





24 hrs. 



66 



209 



37 





48 hrs. 



122 



870 



73 





72 hrs. 



198 



1278 



49 





96 hrs. 



141 



878 



25 



i Bloor, W. K., Pelkan, K. F., and Allen, D. M., J. Biol. Chcm., 1922, lii, 



191. 



