PXEUMOCOCCUS 



in three instances. New bacterial preparations were made from 

 each strain and the nitrogen contents were determined. The 

 results of the second series showed but slight variation from the 

 first. We are inclined to the view that either a relatively high 

 or a low nitrogen content of certain strains may be an inherent 

 quality, slightly deviating in this respect from the average strain, 

 when grown under parallel conditions. Group III strains 

 seemed to fall into two subgroups when based upon their nitro- 

 gen content; one (a) subgroup (four strains) showed an approx- 

 imately lower percentage of nitrogen than the second (b) sub- 

 group (six strains), which showed a nitrogen content similar to 

 the three other serological groups. A number of possible factors 

 which might cause this variation have been considered, but as 

 yet, no one has proved to be the direct cause. Averages of the 

 results taken from ten strains analyzed in each of the serological 

 groups gave the following total nitrogen content : 



Group I, 9.4 per cent.; Group II, 10 per cent.; Group III, 8 

 per cent, (subgroup "A" gave 7.43 per cent.; subgroup "B" gave 

 9.3 per cent.) : Group IV, 8.8 per cent. As is apparent, the total 

 nitrogen content of the four general serological groups does not 

 greatly deviate from a mean average of 9 per cent., a mean 

 which is reduced by using a general average of 8 per cent, for 

 Group III. It must be emphasized that these values are not con- 

 sidered fixed, since a parallel series might slightly alter the per- 

 centage results. 



2 72 (2232) 



The transmission of the virus of herpes febrilis along sensory 

 nerves with resulting unilateral lesions in the central 

 nervous system in the rabbit. 



By ERNEST W. GOODPASTURE and OSCAR TEAGUE. 



[From the Williams H. Singer Memorial Research Laboratory 

 Allegheny General Hospital, Pittsburgh, Pennsylvania.] 



Rabbits inoculated upon the cornea of the right eye with our 

 virus of Herpes febrilis show constantly on about the fourth 

 or fifth day a turning of the head toward the right side; during 

 the succeeding days the neck is twisted strongly toward the 



