Carcinoma. 



33 



Abstracts of the Communications, Pacific Coast Branch. 



Fifth meeting. 

 San Francisco, California, October 7, IQ14. 

 19 (95i) 



Note on the ree'stablishment of a tendency to metastasize in a 

 Flexner-Jobling carcinoma. 



By Theo. C. Burnett (by invitation). 



[From the Rudolph Spreckels Physiological Laboratory of the Uni- 

 versity of California.] 



Owing to a misunderstanding on the part of the attendant, 

 our strain of Flexner-Jobling tumor, which had been carried 

 through nine generations since our experiments with cholesterol, 1 

 was destroyed during the Christmas holidays (1913). In order 

 to repair this serious loss, we asked Dr. Peyton Rous for a new 

 supply, that we might have tumors for future work. He very 

 cordially responded to our demands, as was to be expected, but 

 informed us that his strain had never metastasized, and expressed 

 doubt as to the value of the tumors on that account. 



February 21, 1914, we inoculated forty-seven white rats with 

 the new tumor, which was Rous's twenty-first generation, new 

 series. On March 14, thirty-nine, or eighty-three per cent., had 

 well-marked tumors. Twenty-six of these were kept as "stock" 

 and also served as controls, while thirteen were injected four times, 

 at intervals of two or three days, with one cubic centimeter of a 

 2 per cent, emulsion of cholesterol. The injection was made on 

 the opposite side to that of the tumor inoculation. By April 1 1 

 the tumors began to break down, and we were therefore obliged 

 to kill some of the animals. By May 15 all had been killed. A post 

 mortem was made on each rat, and the results were as follows: 



Control rats 26 killed, 9 metastases; 38.5 per cent. 



Treated rats (cholesterol).. .12 killed, 9 metastases; 75 per cent. 



A portion of one of these metastases was fixed and sectioned in 



1 Robertson and Burnett, Jour. Exp. Med., Vol. 17, No. 3, 1913, p. 344. 



