Acidosis in the Phlorhizinized Dog. 



45 



in the circulatory collapse in poisoning from diphtheria toxin is 

 the arteriole and capillary dilatation. 



27 (959) 



The influence of feeding upon acidosis in the phlorhizinized dog. 



By Stanley R. Benedict and Emil Osterberg. 



[From the Department of Chemistry, Cornell University Medical 

 College, New York City.} 



Experiments have been reported by Geelmuyden 1 and by Baer 2 

 in which they fed protein to dogs under the influence of phlorhizin, 

 and noted a marked drop in the quantity of acetone bodies elimi- 

 nated in the urine. Lusk 3 has called attention to the fact that 

 the animals used by Geelmuyden and by Baer in their experiments 

 were for the most part, only partially phlorhizinized, and that 

 their results might be explained on the assumption that the 

 protein ingested gave rise to dextrose which was burned in the 

 organism. 



In five experiments which we have carried out upon dogs 

 phlorhizinized according to the method of Coolen, we have found 

 a drop of from fifty to ninety per cent, in the quantity of acetone 

 and of oxybutyric acid eliminated, following the ingestion of 

 moderate amounts of protein. A determination of the glucose 

 to nitrogen ratio in the urine showed that the fall in acidosis cannot 

 be accounted for by ascribing it to sugar burned in the organism. 



Since acidosis in the phlorhizinized dog can be practically 

 abolished by such an apparently unrelated factor as the ingestion 

 of protein it is obvious that caution should be used in interpreting 

 results of acidosis studies upon phlorhizinized animals. 



1 Geelmuyden, Zeitschr.f. physiol. Chem., 26, p. 381, 1898. 



2 Baer, Arch.f. exp. Pathol, u. Pharm., 51, p. 271, 1904. 



3 Lusk, Ergebnissee der Physiologie, 1912, XIII, p. 371. 



