58 



Scientific Proceedings (63). 



on a regular slide for study with dark field illumination. In 

 essentially the same way pieces of tissue were placed in plasma 

 under a cover glass and sealed. Precautions to secure aseptic 

 conditions were taken. 



This method keeps trypanosomes living, growing and dividing, 

 and thus many of the stages described by various authors either 

 in the host itself or in the transmitter have been studied in vitro. 

 It is evident that this method may be employed not only for blood 

 parasites but for all protozoan forms which are parasites in cells 

 and thus affords another method of approach for the solution of 

 the complicated life cycles of parasitic Protozoa. 



37 (969) 



The influence of depancreatization upon the state of glycemia 

 following the intravenous injection of dextrose in dogs. 



By I. S. Kleiner and S. J. Meltzer. 



[From the Department of Physiology and Pharmacology of the Rocke- 

 feller Institute for Medical Research.] 



In former experiments 1 it was shown that after the intra- 

 venous injection of large amounts of dextrose (4 g. per kilo) into 

 dogs the sugar rapidly disappears from the blood stream so that 

 after i}4 hours after the end of the injection the blood-sugar falls 

 nearly to its original figure. In the present experiments the same 

 procedure was carried out on completely depancreatized dogs. 

 In these cases the blood-sugar did not fall to its original value or 

 near it; at the end of l}4 hours it was on the average more than 

 twice as high. The following is a comparison of the average 

 figures : 





Blood-sugar. 



Dextrose in Urine, 

 Per Cent, of Amount 

 Injected. 



Before 

 Injection. 



End of 

 Injection. 



ij Hours After 

 End of 

 Injection. 



Normal (5) 



0.20 



O.79 



O.27 



>43% 



Depancreatized (9) . 



O.38 



1. 19 



0.86 



49% 











(uncorrected for 











"diabetic" sugar). 



1 See Proceedings of the American Physiological Society, Vol. 33, 1913, p. xxvii. 



