SCIENTIFIC PROCEEDINGS. 



Abstracts of Communications. 

 Sixty-fourth meeting. 

 47 (979) 



The estimation of " acid-soluble " (inorganic?) and lipoid phos- 

 phorus in small quantities of serum. 



By Isidor Green wald. 



[From the Harriman Research Laboratory, Roosevelt Hospital, 



New York.] 



Phospholipins are quantitatively precipitated with the proteins 

 when blood or serum is added to nine parts of a solution containing 

 one per cent, each of acetic and picric acids. The precipitate 

 does not absorb phosphates. This procedure has been combined 

 with the Neumann method of oxidation and the method of Pouget 

 and Chouchak 1 for the estimation of phosphoric acid for the purpose 

 of estimating the "acid-soluble" and the lipoid phosphorus in 

 small quantities of serum (one cubic centimeter or less) . The results 

 agree with those previously obtained by other methods upon large 

 amounts of serum. 2 In nephritis, the "acid-soluble" phosphorus 

 may be increased to as much as five times the normal amount. It 

 may also be slightly increased after the ingestion of meat. 



1 Pouget and Chouchak: Bulletin societe chimique (4), 5, 104, 1909; 9, 649, 191 1. 



2 Greenwald : Journal of Biological Chemistry, 14, 369, 1913; American Journal 

 of the Medical Sciences, 147, 225, 1914. 



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