136 



Scientific Proceedings (66). 



Treatment was begun seventeen days after inoculation with 

 the tumor, except in the cases of a few animals which were treated 

 on the tenth day, and of a few which were first rendered diabetic 

 and then inoculated. None of the tumors at the beginning of 

 treatment had reached the size of the largest tumors reported 

 cured by Benedict and Lewis. 



Of the rats, there were alive twenty-four days after inoculation 

 and seven days after beginning of treatment, 81 treated animals 

 and 89 controls. Of the treated rats, 37 per cent, showed partial 

 or subsequently complete absorption, while 58 per cent, of the con- 

 trols had undergone spontaneous absorption. The largest tumor 

 to disappear was among the controls and measured 26 X 14 mm. 

 The largest growth noted occurred among the animals rendered 

 diabetic before inoculation. 



The laboratory stock of Buffalo rat sarcoma for the past 

 fifteen months, representing over five hundred tumors, has showed 

 40 per cent, spontaneous absorption. 



The mice with the transplantable carcinoma showed no cases 

 of absorption, and those treated did not differ from the controls 

 except in the death rate. The spontaneous tumors were not 

 affected by the treatment. 



In view of the great tendency of the Buffalo rat sarcoma to 

 undergo spontaneous absorption and the failure of the treatment 

 in progressively growing tumors, the results of Benedict and Lewis 

 must be considered as due to spontaneous absorption rather than 

 to the effect of induced diabetes. 



82 (1014) 



A new principle in isolation of spirochetes in pure culture. 

 By J. Bronfenbrenner. 



[From the Pathological and Research Laboratories of the Western 

 Pennsylvania Hospital, Pittsburgh, Pa.] 



In attempting to obtain pure cultures of spirochetes from the 

 lesions of rabbits showing symptoms of generalized lues, I was 

 confronted with extreme difficulties in the case of two strains 



