146 



Scientific Proceedings (67). 



The theory involved in this procedure is as follows: The 

 urotropin in contact with acid or acid phosphate breaks up into 

 formaldehyd and ammonia. The ammonia thus liberated is 

 available for neutralizing the acid injected, so that an animal that 

 has received urotropin should be more resistant to the harmful 

 effects of the injected acid than one that has not received urotropin. 

 In round numbers, 1 gram of urotropin will liberate 1 gram of 

 formaldehyd and sufficient ammonia to neutralize I gram of hy- 

 drochloric acid. 



The results obtained thus far are not conclusive. In a total 

 of 10 experiments on 4 cows that received both acid and urotropin, 

 (see accompanying table) formaldehyd was detected in the milk 

 4 times and was not detected 6 times. The positive findings are 

 tentatively regarded as correct. In some cases, the amounts of 

 hydrochloric acid that could be injected into cows without ap- 

 parent injury, were so unexpectedly large, that the results are 

 presented now because of their possible interest to those studying 

 acidosis or the adaptibility of the vascular system. 









Injected Intra- 



Uro- 

 tropin 











Body 

 Weight, 

 Kilos. 



venously. 



Formal- 



Grams HC1 per Kilo 

 Body Weight. 



Animal. 



Date. 



Liters. 



Per 



Fed by 

 Mouth, 



dehyd in 

 Milk. 







Cent. 



Grams. 















HC1. 







Cow 715 



2/15 



461 



6 



0.6 



1.350 



Absent 



0.08 





2/17 





13.5 



0.6 



1.350 



Absent 



0.18 



Cow 1009 . . . 



3/13 



2S0 



15-5 



0.6 



None 



No 



0.37 





3/20 



209 



20 



0.9 



627 



Milk 



0.87 (after 48 hour 

 fast) 



Cow 1013 . . . 



i/iS 



293 



"•5 



0.6 



80 



Absent 



0.23 





1/16 





12 



0.6 



80 



Absent 



0.25 





1/27 





20 



0.9 



907 



Present? 



0.61 





3/27 



227 



2-5 



0.6 



570 



Absent 



0.07 killed (had fasted 

 48 hours) 



Cow 1026 . . . 



2/10 



304 



3 



0-9 



900 



Present? 



0.09 killed 



Cow 1042 . . . 



1/20 



231 



12 



0.9 



80 



Absent 



0.47 





4/1 



239 



8.4 



0.9 



478 



Trace 



0.32 killed 



Cow 1096. . . . 



2/1 



391 



4.4 



0.9 



None 





0.10 killed 



Steer 1128 . . 



2/5 



339 



10 



0.9 



None 





0.26 





3/i7 





31.7 



0.6 



None 





0.56 





3/24 



300 



23 



0.9 



900 





0.70 killed (had fasted 

 48 hours) 



Cow 852. , 



4/7 



386 



9.2 



0.9 



772 



Present 

 1 :ioo 000 



0.22 killed 



The animals varied greatly in their tolerance for the injected 

 acid. Thus Cow 1026 was killed by a single injection of 3 liters 



