Anaphylaxis to Formed or Cellular Elements. 147 



of 0.9 per cent, hydrochloric acid: Steer 1128 was killed by the 

 third injection of 23 liters of 0.9 per cent, acid; while Cow 1009 

 still lives after receiving the unexpectedly large amount of 20 liters 

 of 0.9 per cent, acid following an injection of 15.5 liters of 0.6 per 

 cent, acid made seven days before. In Szili's work, the largest 

 dose of acid tolerated by the experimental animal (a ram) was 

 0.23 gram of hydrochloric acid per kilo of body weight. In the 

 present work, Cow 1009 is apparently in good condition after re- 

 ceiving 0.87 gram of hydrochloric acid per kilo of body weight. 



88 (1020) 



Anaphylaxis to formed or cellular elements. 



By Richard Weil and B. S. Denzer. 



[From the Department of Experimental Therapeutics, Cornell Medi- 

 cal School.] 



Our present knowledge of anaphylaxis is based almost entirely 

 on the study of proteins in solution, such as blood serum. From 

 the standpoint of infectious disease, the analysis of the immunolog- 

 ical reaction to formed elements would appear to be of greater im- 

 portance. The anaphylactic response to bacteria has regularly 

 been found to be extremely slight. The present report deals with 

 a study of the anaphylactic response to red blood cells. 



Friedberger reported during the current year that guinea pigs 

 could not be sensitized to alien red blood cells either by the active 

 or by the passive method. On the contrary, these animals can 

 regularly be sensitized by either method provided the proper 

 technique be followed. In order to sensitize actively against 

 alien red blood cells it is essential to give a series (2 or 3) of pre- 

 liminary injections, instead of the single sensitizing injection which 

 is customary in the case of serum. The reason for this will be 

 obvious from the subsequent data. As regards passive sensiti- 

 zation, it is of importance to note that Friedberger, like Thiele 

 and Embleton and others who have worked on this subject, used 

 the serum of rabbits immunized against sheep red blood cells. 

 This particular type of serum, however, is peculiarly unfitted 

 for such an experiment. It possesses primary toxicity for the 



