160 Scientific Proceedings (67). 



larger masses into small fragments which then were scattered 

 through the protoplasm of these cells. The intracellular position 

 of the fat could be observed during all stages of the experiment. 

 In other instances, the phagocyted fat was removed from the 

 blood channels and passed to the lymphatics. Such fat then 

 appeared to lie free and not within cells. In the arterioles the 

 fat appeared both in the proliferating endothelial cells as well as 

 in deeper portions of the intima and media. In the latter structure 

 it was commonly observed as an extracellular deposit. It was 

 not uncommonly seen that the endothelial cells contained both 

 fatty masses and cholesterin crystals. These crystals lay in clefts 

 quite apart from the oil globules. 



By staining with sudan all the oil masses were equally colored. 

 Sections stained with Nile blue sulphate showed the presence of 

 neutral fats, fatty acids, and intermediate mixtures of these. 

 With the polarizing microscope some anisotropic globules could 

 be seen, while free cholesterin plates were also demonstrated. 

 The majority of the fatty acid globules were intracellular. These 

 were usually smaller than the globules of neutral fat. The fatty 

 acid racidal was also demonstrated by the Fischler method. 



Associated with the intra- and extra-cellular fatty acid globules, 

 was found the deposition of calcium salts. The calcium became 

 precipitated in the borders of these fatty acids and gradually 

 irregular calcium precipitates encroached upon the center of the 

 acid globule. This deposit took place in the protoplasm of the 

 large phagocytic cells as well as in the fatty deposits which were 

 lying free in the tissue or in the lymph channels. At the end of 

 eight weeks we found the lung substance filled with these minute 

 calcareous masses lying in areas of cell proliferation looking not 

 unlike small tubercles. The calcareous process was also recognized 

 in the walls of the blood vessels. In the latter, the process 

 occurred in the deep intima and media where fatty deposits were 

 also demonstrated. These calcium salts were recognized by 

 staining with hematoxylin and their phosphatic radicals with silver 

 nitrate. They could be removed by treating the tissue with 

 hydrochloric acid. 



By the method here employed one is able to demonstrate the 

 intimate association between the abnormal presence of fats and 



