172 



Scientific Proceedings (67). 



account had been paid to the order in which the animals had been 

 treated it was only possible to ascertain the numbers of the six 

 so treated. From the time necessary, however, for the injections 

 the animals must have died from within one to ten minutes after 

 they had received their injection. Following this the dose was 

 halved (i. e., 0.01 c.c. diluted to 0.5 c.c.) for the remaining 66 

 animals without a casualty or any signs sufficiently definite that 

 anaphylactic intoxication could with certainty be diagnosed. 

 It so happened that the six animals receiving the higher dose of 

 0.02 c.c. were all from the set of 42. 



The second injection (subcutaneous) was given shortly after 

 completing the first and 0.1 c.c. protein (diluted to 0.5 c.c. with 

 saline) was tried. As before two acute deaths occurred in the six 

 so inoculated. Again the dose was halved, as in the preceding 

 injections, without any fatality for the now remaining 64 animals. 

 This time it so happened that five of the six guinea-pigs, which 

 had received the larger dose, belonged to the set of 30, and both 

 deaths occurred amongst the five, so that there was no evidence 

 that the character of the initial sensitizing dose had had any 

 bearing on the results. 



In all four cases death was acute and rapid; the post mortem 

 showed the typical emphysematous condition of the lungs. 



The third (subcutaneous) desensitizing dose of 0.125 c.c. 

 protein (diluted to 0.5 c.c. with saline) was given at 11 A.M., 

 and the fourth (subcutaneous) of 0.5 c.c. protein at 4.30 P.M. of 

 the following day, March 25, without any immediate ill effects. 

 The following morning, however, one animal in the set of 42 was 

 found dead. The post mortem showed a most marked condition 

 of general anasarca. On cutting the skin the subcutaneous tissues 

 were very swollen and fluid rapidly oozed from the cut surface, 

 the serous cavities contained a very appreciable amount of free 

 fluid, the lungs were very edematous and the tubes greatly 

 distended with clear fluid. 



Following the two injections of the 25th of March, large doses 

 were given on subsequent days without any evidence of toxic 

 symptoms. 



Besides the two sets of guinea pigs above, there was also a 

 small set of six which had received an initial dose of 0.5 c.c. goat 



