A Note on the Desensitization of Guinea-Pigs. 173 



serum on January 22, 1915. Reinjection was made on March 24 

 of 0.01 c.c. and 0.1 c.c. (diluted to 0.5 c.c. with saline) without 

 any ill results. Further injections of larger amounts gave negative 

 results. 



These results on guinea-pigs might be gathered together as 

 follows: 



1. While the first desensitizing dose was sufficiently strong to 

 cause acute death in two out of the six guinea-pigs, one half the 

 dose failed to cause any marked or certain evidences of anaphy- 

 lactic poisoning in the remaining 66 animals. 



2. While the strength of the second desensitizing dose was 

 sufficiently strong to cause acute death in two out of six guinea-pigs, 

 one half the dose again failed to cause marked or certain evidences 

 of an anaphylactic poisoning in the remaining 64 animals. 



3. Two subcutaneous desensitizing doses were given on two 

 successive days and this proved successful in producing a fully 

 refractive state in those that survived. 



4. On the night following the fourth dose, which was 0.5 c.c. 

 protein, one pig died and the post mortem showed a marked 

 condition of general anasarca. 



5. All injections were made subcutaneously and into approxi- 

 mately the same area. 



6. Out of 72 guinea-pigs, previously injected with bovine 

 protein a total of 5 died during the course of desensitization, four 

 in acute anaphylactic shock and one later with general anasarca. 



7. Six guinea-pigs showed no little evidence that they were 

 sensitive to goat serum. 



CHART 1. 



Illustrating First and Second Desensitizing Doses on Guinea-Pigs, 50 Days 

 After Last Initial Injection. 



Time and Date. 



First Reinjection 

 Subcutaneously 10:30 

 a. m . March 24. 



Anaphy- 

 lactic Death 

 in 



Second Reinjection 

 Subcutaneously 11:45 

 a. m., March 24. 



Anaphy- 

 lactic Death 

 in 



Set of 30 guinea-pigs 

 Set of 42 guinea-pigs 



Dose .02 c.c. for 0 

 Dose .01 c.c. for 30 

 Dose .02 c.c. for 6 

 Dose .01 c.c. for 36 



0 

 0 

 2 

 0 



Dose 1 c.c. for 5 

 Dose .05 c.c. for 25 

 Dose .1 c.c. for 1 

 Dose .05 c.c. for 39 



2 

 0 

 0 

 0 



