Anaphylactic Reaction in Smooth Muscle. 175 



For extracting the amines from a sodium carbonate solution 

 free from alcohol, a mixture of 1 part amyl alcohol with 3 parts 

 ether was used, as it is a better solvent than ether alone and does 

 not emulsify like amyl alcohol alone. The amines were recovered 

 by extracting the ether-amyl alcohol mixture with dilute H2SO4. 



From the fresh plants of Phoradendron flavescens, var. macro- 

 phylla and Phoradendron californicum (obtained through the 

 courtesy of Prof. J.J. Thornber, University of Arizona) no amines, 

 precipitable by oxalic acid from ether solution, were obtained. 

 However, they will be examined further. 



105 (1037) 



On the mechanism of the anaphylactic reaction in smooth muscle. 

 (Preliminary communication.) 



By William H. Moore (by invitation). 



[From the Department of Bacteriology and Immunity, Leland 

 Stanford Jr. University.] 



If the uterus of a sensitized guinea-pig is rendered bloodless 

 by transfusing it with Locke's solution, the sensitiveness of the 

 uterine muscle to the foreign proteid is increased. 



If the muscle is further freed from tissue lymph and from other 

 diffusible tissue elements by repeated centrifugation in Locke's 

 solution, the sensitiveness is greatly decreased. 



If the serum and diffusible tissue elements thus removed are 

 replaced in the muscle by repeatedly centrifuging the muscle in 

 dilute normal guinea-pig serum, the sensitiveness is restored 

 quantitatively. 



This would seem to indicate that the reaction of the anaphy- 

 lactic uterine muscle is dependent upon two factors: (a) a factor 

 induced in the fixed cells by the process of sensitization, and (b) 

 some normal serum component. It would also indicate the pres- 

 ence of a third element, (c) an antitoxic or anti-anaphylactic 

 serum component. 



