Inhibition of Sodium Oleate Hemolysis. 199 



change is an increase of the globulins and a decrease of the albu- 

 mins. Such being the case, it seemed of interest to ascertain 

 whether these modifications affected the rate of coagulability of 

 the blood. Accordingly, the blood of a number of horses which 

 have been immunized for months or years for the purpose of 

 obtaining antisera against diphtheria, tetanus, meningitis, etc., 

 was investigated from this point of view. Oxalated plasma was 

 employed in the coagulation tests. The accompanying table 

 gives the results of this investigation, and shows that the coagula- 

 tion-time remains fairly constant in spite of marked variations in 

 the proteins, that it is a factor which is not readily disturbed. 



Horse No. 



Total Protein. 



Euglobulin. 



Pseudo- 

 globulin. 



Albumin. 



Coagulation 

 Time. 



414 



6-93 



• 78 



2.74 



3-27 



14 min. 



508 



6.15 



•30 



3-58 



2.15 



14 *' 



536 



7-49 



•54 



4.08 



2.66 



12 " 



542 



6.49 



.60 



3.61 



2.15 



14 " 



566 



7.22 



•53 



2.98 



3-59 



7 



593 



6-93 



•53 



307 



3-21 



12 " 



596 



7.20 



.76 



2.90 



3-37 



14 " 



N. 1 



7.17 



.61 



3-42 



2-97 



12 " 



N. 2 



6.70 



.48 



305 



2.98 



15 " 



121 (1053) 



Inhibition of sodium oleate hemolysis and toxicity by cholesterin. 



By Oskar Klotz and May E. Bothwell. 



[From the Pathological Laboratories, University of Pittsburgh, 

 Pittsburgh, Pa.] 



The hemolytic quality of soap solutions and particularly of 

 sodium oleate has been studied by a number of investigators 

 (Sachs, Meyer, Moore). Meyerstein later showed that the 

 oleate hemolysis could be inhibited by lipoids, cholesterin, serum 

 and organ extracts. Sodium stearate and palmitate are less 

 active than the oleate. Moore has shown that the laking qualities 

 of the soaps are in proportion to the unsaturated bonds of the 

 fatty acids and that by iodine saturation can be inhibited. 



The toxicity of soaps was studied by Munk who found that 

 the quantity equal to O.ll to 0.13 gram oleic acid per kilo body 



