Inhibition of Sodium Oleate Hemolysis. 201 



to saturation. In this state, however, the compound is very- 

 gelatinous and hard to handle, while the addition of water for 

 further dilution again leads to dissociation products. 



Animals treated with the cholesterin sodium oleate mixture 

 show no evidence of excretion of these substances in the urine. 

 The compound readily circulates in the blood and is not filtered 

 out by the capillaries. The lungs are quite free from change. 

 We have noted, however, that frozen sections of formalin-fixed 

 tissue showed anisotropic globules and an unusual amount of (?) 

 cholesterin spicules in the liver parenchyma. The liver appeared 

 quite yellow and fatty but the fat did not exist as the coarse 

 globular fat of fatty infiltration. In these experiments we did not 

 find the enlarged fatty adrenals as were present in another series 

 in which the cholesterin materials were fed to the animals. The 

 fate of the cholesterin and soaps has up to the present time not 

 been determined. 



The same cholesterin sodium oleate emulsion was used to 

 demonstrate the inhibitory qualities of cholesterin upon soap 

 hemolysis. It was found that 0.05 c.c. of a 5 per cent, solution 

 of sodium oleate would hemolyze 1 c.c. of a 1 per cent, suspension 

 of human blood cells in less than 18 hours at room temperature. 

 On the other hand, 1.2 c.c. of the cholesterin sodium oleate com- 

 pound did not hemolyze a similar quantity of blood. It was like- 

 wise found that the addition of normal serum to any mixture of 

 sodium oleate and cholesterin still further inhibited the hemolysis 

 and toxicity of the soap. 



It is quite easy to prepare solutions of soap containing different 

 quantities of cholesterin and by this means, observe the inhibitory 

 influences of this substance. We have found that the 5 per cent, 

 solution of sodium oleate in saline with the addition of 5 per cent, 

 pure cholesterin is the most useful for study and is easily handled. 

 The histological changes in tissues resulting from abnormal quan- 

 tities of fluid cholesterin compounds will be reported upon later. 

 By this combination of soap and cholesterin we have a means of 

 introducing this otherwise inert substance in a fluid state which is 

 assimilable. 



