14 



Scientific Proceedings (69). 



in different dogs between 0.5-3.5 m g- NH 3 per 100 c.c. of juice, 

 there being some variation from day to day, and at different 

 times during the same day in the same dog. The juice was 

 collected for a period before feeding and one-hour periods after 

 feeding. The addition of NH 4 C1 to the dogs' food caused in each 

 instance an increase in the NH 3 -content of the gastric juice col- 

 lected from the Pawlow pouch. Three dogs, in which gastric 

 ulcers had been produced experimentally showed an increase in 

 the NH 3 -content at irregular intervals. After a time two of 

 these dogs refused to eat and the juice collected during this time 

 showed a progressively increasing content of NH 3 . At autopsy 

 these dogs showed either active or healed ulcers, usually located 

 in the Pawlow pouch. 



b. The experiments on normal healthy individuals were con- 

 ducted on men who were connected with the laboratory. The 

 juice was collected by means of the Rhefus stomach tube before 

 and after feeding on Ewald meal. With three exceptions the 

 NH 3 -content of the juices from these men ranged between 0.5-3.5 

 mg. of NH 3 per 100 c.c. of juice. In these three men the NH 3 - 

 content ranged between 10-15 m g- NH 3 per 100 c.c. of juice. 



A series of experiments was then conducted on one of these 

 men with high NH 3 -content. The diet was so arranged that there 

 were periods of low protein and of high protein ingestion, and the 

 NH 3 content of the gastric juice and the total NH 3 of each day's 

 urine were noted. During low-protein ingestion the NH 3 content 

 of the gastric juice fell in five days from 12-3.5 m g- an d the NH3 

 of the urine also showed a marked decrease. During the high- 

 protein ingestion the NH 3 -content of the gastric juice rose in 3 

 days from 12-28 mg. NH 3 and the NH 3 of the urine also showed 

 a marked increase. In both cases the NH 3 in the gastric juice 

 and in the urine came back to the original level within two days 

 after resuming the usual diet. 



In the second experiment the diet was kept uniform through- 

 out the period and during certain periods an excess of alkalis or 

 of acids was added. During the period of excess alkali ingestion 

 the NH 3 of the gastric juice remained the same while the total 

 NH 3 of the urine fell to 1 /10 its former level. During the ingestion 

 of acid the NH 3 of the gastric juice again remained the same while 



