A Qualitative and Quantitative Test for Levulose. 71 



A glance at the above table will suffice to show that the flow 

 of urine bears but slight relation to the degree of dilution of the 

 blood as indicated by the specific gravity determinations. Di- 

 uresis is apparently the greatest when the percentage of blood 

 sugar is highest. On the other hand, there is no question but 

 what diuresis is absent in experiments showing excessively high 

 blood sugar. 



For the experiments of May 27 and 28, and of June 4 and 5, 

 the same animals were used, the experiments differing only in 

 that the rate of injection was rapid in one case and slow in the 

 other. Here it seems that the presence of diuresis depends upon 

 the rapidity of injection, and not upon the blood sugar content, 

 which in both cases is excessively high. 



III. The quantity of sugar per c.c. of urine was surprisingly 

 constant when the blood sugar was above 0.4 per cent., i. e., during 

 the period following injection. In a series of experiments with the 

 blood sugar varying from 0.4 to 0.6 per cent, the sugar per c.c. 

 of urine varied from .045 to .052 gm., independently of the presence 

 or absence of diuresis. Toward the end of the experiment, how- 

 ever, with blood sugar falling, and diuresis coming to an end, the 

 sugar per c.c. of urine rises steadily. For example, in one experi- 

 ment, at different periods, the blood sugar percentage was .5, .35, 

 and .28; while for corresponding periods the urine sugar per c.c. 

 was .05, .07, and .08 gm. 



42 (1106) 



A method for the estimation of levulose in presence of glucose. 



By Leon Loewe (by invitation). 



[From the Department of Chemistry, Cornell University Medical 

 College, New York City.] 



The reagent consists of 0.2 per cent, aqueous solution of orcein 

 (Kahlbaum) and 85 per cent, phosphoric acid (Eimer and Amend) 

 in separate containers. 



The qualitative test is carried out as follows: to 1 c.c. of the 

 solution under investigation in a test tube, add from 6 to 8 drops of 

 the orcein solution and 1 c.c. of the phosphoric acid. The test tube 



