Effect of Temperatures on Formation of Hemolysins. 97 



calculation of averages by calling this figure 10 when we only 

 know that it was greater than 10 will not materially affect the 

 results. Titrations were not performed during the second week 

 of Series II. Series I and III were stopped after six weeks. 

 Series V after five weeks and Series IV after four weeks. Other 

 blanks in the table are due to the death of the animals. 









Hemolytic Power of Serum. (Cubic Millimeters of Serum 



Series. 



Rabbit. 



Air 







Necessary to Hemolyze. ) 







Temperature. 



1 



Week. 



2 



Weeks. 



Weeks. 



Weeks. 



5 



Weeks. 



6 



Weeks. 



7 



Weeks. 



I 



I 



30° 



10.000 



.118 



.070 













2 



30° 



•500 



.083 



.070 



•°59 



.044 



.022 







51 



20° 



10.000 



.161 



.073 













52 



20° 



10.000 



.069 



.050 



.060 



.105 



.089 





II 



186 



30° 



•145 





.069 



.020 



.040 



.047 



.067 





187 



30° 



.100 





.036 



•034 



.032 



•054 



.084 





183 



20° 



.178 





•075 



.014 



.040 



■055 



.067 





I84 



20° 



.189 





.024 



.040 



.020 



•033 



.061 



III 



70 



io° 



.588 



.060 



.028 



.025 



.029 



•033 







75 



30° 



1. 000 



.067 



.042 



.022 



•033 



.040 







86 



20° 



•213 



• 050 



.024 



.020 



• 025 



.029 







no 



20° 



.500 



.067 



.029 



.017 



.022 



.028 





IV 



88 



30° 



.588 



•075 



.020 



.026 











171 



30° 



.588 



.069 



.025 



.032 











4 



20° 



.500 



.027 



.020 



.026 











191 



20° 



.200 



•044 



.015 



.020 









V 



72 

 77 



30° 



30° 



10.000 

 10.000 



10.000 

 .100 



.172 

 .164 













100 



30° 



10.000 



10.000 



10.000 



.200 



•044 









173 



30° 



.588 



.400 



10.000 



.O63 



.044 









83 



20° 



.123 



.062 















136 



20° 



.238 



.270 



10.000 



.238 



.083 









147 



20° 



•833 



.097 



.076 



.110 



.083 









148 



20° 



.588 



.588 



.161 



.047 



.040 







General average . 



30° 



3.670 



2.100 



1.720 



■053 



.038 



•039 



.076 







20° 



1.970 



.144 



•958 



•059 



.052 



.047 



.064 



The results as presented in the table appear to indicate a 

 distinct decrease in the rate of hemolysin formation on the part 

 of the heated rabbits. The hemolytic power of the blood of 

 individual animals of course varies within wide limits, yet the 

 averages show that in order to produce hemolysis it was uniformly 

 necessary to use larger quantities of serum from the heated rabbits 

 during the first three weeks. The influence of heat appears to 

 show itself in a delayed formation of hemolysins rather than in a 



