Separation of Serum. 



125 



of the labyrinth, as compared with the deportment of cats or dogs 

 after loss of one labyrinth. 



Two cats were subjected to experimental ablation of the vermis 

 and left lateral lobe of the cerebellum. The eye movements were 

 different from those following labyrinthine lesions. One marked 

 motor defect was the trembling and uncertainty of movement of 

 the head when attempting to take food. Two different stages in 

 recovery from the effects of the cerebellar lesion were shown, in 

 one of the cats, with the gradual amelioration of the symptoms in 

 the second stage taken at an interval of about one month after the 

 first. 



The film of the rabbit was made through the courtesy of Pathe 

 Freres. The films of the cats were paid for out of the Patton 

 Fund of Northwestern University Medical School. 



69 ("33). 



A separation of serum into coagulative and non-coagulative 



fractions. 



By Alfred F. Hess. 



[From the Research Laboratory, Department of Health, New 



York City.] 



As is well known, it is possible, by means of salting out with 

 appropriate percentages of ammonium sulphate or sodium chloride, 

 to almost entirely separate the albumin from the globulin and the 

 pseudo-globulin of serum. This has been done in the preparation 

 of diphtheria antitoxin, where it has been found that the antitoxin 

 is closely associated with the pseudo-globulin fraction. 



A similar procedure was carried out to determine the associ- 

 ation of the coagulative principles of the serum. It was found that 

 in human plasma as well as in horse plasma, these substances are 

 linked with the euglobulin fraction. If these three proteid frac- 

 tions of the serum are separated and dissolved in normal salt 

 solution and added to plasma (with the addition of a small amount 

 of calcium) the euglobulin will markedly hasten coagulation, 

 whereas the two other fractions will have either no effect or a 

 slightly inhibitory action. It is possible in this way to prepare a 



