Protein Absorption by Blood Corpuscles. 173 



96 (1160) 



Protein absorption by blood corpuscles. 



By W. H. Manwaring and Yoshio Kusama. 



[From the Department of Bacteriology and Immunity, Leland Stan- 

 < ford, Jr., University.] 



If 1 per cent, goat serum is added to freshly drawn defibrinated 

 normal rabbit blood, the mixture incubated for one hour, and then 

 separated by centrifugation into serum and corpuscle fractions, 

 a titration of the serum fraction by specific precipitin methods 

 will usually show but 25 per cent, of the goat protein originally 

 added to the blood. 



If the serum and corpuscle fractions so obtained are allowed 

 to undergo independent autolysis (10 hrs., 37 0 C), a distinct 

 restoration of the goat protein is observed in each fraction. The 

 restoration of the protein in the corpuscle fraction, however, is 

 usually much more pronounced than that in the serum fraction, 

 and may amount to as much as 50 per cent, of the total protein 

 originally added to the blood. 



If goat serum is slowly injected intravenously into normal 

 rabbits in amounts not exceeding 1 per cent, of the total blood 

 volume, and blood is withdrawn from 1 to 4 hours later, a distinct 

 restoration of the goat protein can be brought about by allowing 

 the centrifuged but unwashed corpuscles so obtained to undergo 

 autolysis. 



Parenterally introduced proteins, therefore, are apparently 

 absorbed in large measure by the circulating blood corpuscles. 



97 (1161) 



Toxicity of foreign sera for the isolated mammalian heart. 



By W. H. Manwaring, Arthur R. Meinhard and Helen 



L. Denhart. 



[From the Department of Bacteriology and Immunity, Leland Stan- 

 ford, Jr., University.] 



Seven per cent, to 10 per cent, goat serum in Locke's solution 

 perfused under constant pressure and temperature through the 



