176 



Scientific Proceedings (76). 



Such inactivated anaphylactic sera are strongly antitoxic. 

 The presence of a thermo-stable antitoxin in the unheated ana- 

 phylactic serum is apparently masked by the relatively strong 

 thermo-labile toxin-increasing or toxin-producing substance. 



This thermo-stable antitoxin is present in larger amounts in 

 the sera of rabbits sensitized or immunized by multiple injections, 

 than in rabbits sensitized by a single injection. 



Sera of partially immunized rabbits (3-5 injections) added to 

 the perfusion fluid, usually give a non-fatal shock with normal 

 hearts. The heart may come to a complete standstill by the end 

 of four minutes, may remain inactive 1 for from two to four min- 

 utes, and then recover completely within two or three minutes. 

 A heart that has passed through such a non-fatal shock will 

 usually continue to beat strongly and regularly for an hour or more. 



Sera of highly immunized rabbits (8-12 injections) added to 

 the perfusion fluid, usually give no shock, and show only a marked 

 antitoxic action. 



99 ("63) 



Autolysis of anaphylactic and immune tissues. 



By W. H. Manwaring and Ruth Oppenheimer. 



[From the Department of Bacteriology and Immunity, Leland Stan- 

 ford, Jr., University <.] 



The post-mortem autolysis of normal, anaphylactic and 

 immune guinea pig livers was followed by determining the changes 

 in the relative amounts of coagulable and non-coagulable ni- 

 trogen (Kjeldahl method). The anaphylactic guinea pigs had 

 been sensitized by a single injection of egg-white or goat serum. 

 The sensitizing dose varied from 0.1 c.c. to 2 c.c. Analyses were 

 made from 11 to 17 days after the injection. The immunized 

 guinea pigs had been injected at 4-7 day intervals with from 

 3 to 7 doses of the same antigens. They were analyzed from 

 8 to 12 days after the final injection. A summary of the data so 

 obtained is shown in the following table: 



1 The coronary perfusion is made under constant pressure, and is only partially 

 dependent upon heart action. The perfusion of the myocardium, therefore, con- 

 tinues during the inactive period. 



