i 7 8 



Scientific Proceedings (76). 



If pneumococci are similarly deposited in the liver of an actively 

 immunized rabbit, in the presence of immune rabbit blood, a 

 gradual decrease in the deposited pneumococci is observed. By 

 the end of 5 or 6 hours' incubation, the tissues have usually become 

 relatively sterile. The few remaining microorganisms usually 

 multiply later to form distinct colonies. The microorganisms 

 in the larger hepatic blood vessels, not in contact with the specific 

 parenchyma cells, are not so destroyed. 



This hepatic destruction of the pheumococci is not associated 

 with leucocytic accumulations, nor is it necessarily accompanied 

 by phagocytosis by the endothelial cells. There is apparently 

 an hepatic mechanism in the immune animals for the extra- 

 cellular destruction or digestion of the microorganisms. Pneu- 

 mococci taken up by the endothelial cells are apparently protected 

 to a certain extent from this destruction. 



101 (1165) 



A method for the determination of small amounts of sugar in urine. 



By V. C. Myers. 



[From the Laboratory of Pathological Chemistry, New York Post- 

 graduate Medical School and Hospital.] 



All human urines probably contain small amounts of sugar, 

 as has quite recently been pointed out by both Cole 1 and Folin, 2 

 who have described tests for the detection of this small amount of 

 sugar. It has been found possible to determine this reducing 

 substance by precipitating the creatinine and uric acid, and 

 probably other interfering substances with picric acid as suggested 

 by Folin for his qualitative test, and then employing a technique 

 similar to that introduced by Benedict and Lewis 3 for the esti- 

 mation of the sugar of the blood. 4 It is presumed that the re- 



1 Cole, S. W., Lancet, 1913, II, 861. 



1 Folin, O.. J. Biol. Chem., 1915, XXII, 327. 



3 Lewis, R. C and Benedict, S. R., J. Biol. Chem.. 1915, XX, 61. See also 

 Myers, V. C, and Bailey, C. V., J. Biol. Chem., 1916, XXIV. 147- 



4 In a recent conversation with Professor S. R. Benedict, he informed me that 

 Mr. Oesterberg, of the Cornell Chemical Laboratory, had likewise utilized this- 

 method for urine. 



