Attenuation of Living Agents of Cyanolophia. 189 



the vein is prevented, a comparison of the flow from the adrenals 

 in successive observations is made possible by noting the intervals 

 of time necessary for the pocket to fill up to this point. The 

 quantity of blood required to fill the pocket can be determined 

 once for all in each animal. The vertical position of a portion of 

 the pocket helps to empty it without manipulation when the 

 clamp is removed. 



6. The sensitiveness of the eye-reactions to epinephrin dis- 

 charged from the adrenals, for example in response to stimulation 

 of the splanchnics, can be increased notably by temporarily clamp- 

 ing off alternative arterial paths. This must be done at such an 

 interval of time after the beginning of stimulation as is not more 

 than sufficient to allow the epinephrin to reach the beginning 

 of the aorta. A larger proportion of the blood containing the 

 epinephrin is thus forced to take the path to the eye whose re- 

 actions are being studied. If, for instance, the left iris is the 

 denervated one, clamping at the proper moment of the thoracic 

 aorta and the innominate markedly increases the reaction. It can 

 be further increased by tying off all accessible branches of the left 

 carotid except those through which the eye must obtain its blood 

 supply. 



108 (1172) 



Attenuation of the living agents of cyanolophia. 



By Rhoda Erdmann. (By invitation.) 



[Osbom Zoological Laboratory, Yale University, New Haven, Conn.] 



Paschen 1 (191 1) says that leucocytes must play an important 

 part in the process of immunization. This remark seems partly 

 justified in the attenuation process of cyanolophia. The living 

 agents of cyanolophia are differently affected in tissue cultures 

 of red bone marrow from white bone marrow. 



'Paschen, O., "Handbuch der Teclmik und Methodik der Immunitatsfor- 

 schung," 1911. 



