Scientific Proceedings (76). 



Experiment I, 1915. 



Serum B Taken 36 Hours after Inoculation of Virulent Brain A in a 

 Chicken, Shortly before its Death, Was Inoculated in a Tissue 

 Culture of Red Bone Marrow and Chicken Plasma. 



Length of Time in which Virulent Serum B was 

 Cultivated in Tissue Culture at 30 0 C. 



Record No. 

 of Chicken. 



Date of Death. 



Length of 

 Incubation. 



Nov. 12-Nov. 15 



No. 1 



Nov. 18 



48 hours 



Nov. 12-Nov. 15 



No. 3 



Nov. 17 



38 hours 



Nov. 12-Nov. 18 



No. 2 



Nov. 21 



72 hours 



This proves that the virus can be kept alive six days at a 

 temperature of 38 0 C. in a tissue culture of red bone marrow. 

 Chickens No. 1 and No. 3 died in 48 and 38 hours. Chicken 

 No. 2, which had been inoculated with serum B that had been 

 kept 6 days in the tissue culture, died twenty-four hours later 

 than the chicken which had been inoculated with serum B that 

 had been only three days in a tissue culture of red bone marrow. 

 This proves a certain attenuation by the cultivation of virulent 

 serum in red bone marrow. 



The living agents, which probably cause cyanolophia, can be 

 cultivated in red bone marrow tissue cultures even longer than 

 six days without losing their virulence. 



Experiment XII, 1916. 



Serum V Taken 36 Hours after Inoculation of Virulent Brain U in a 

 Chicken, Shortly before its Death, Was Inoculated in a Tissue 

 Culture of Red Bone Marrow and Chicken Plasma. 



Length of Time in Which Virulent 

 Serum V was Cultivated in 

 Tissue Culture at 38 0 C. 



Record No. of 

 Chicken. 



Date of Death. 



Length of 

 Incubation. 



Feb. 23-March 2 



No. 17 



March 4 



48 hours 



Feb. 23-March 6 



No. 18 



Remained alive 





Feb. 23-March 11 



No. 19 



Remained alive 





So we can keep virulent the living agents of cyanolophia outside 

 of the chicken 6-8 days at 38 0 C, yet in the tissue culture of red 

 bone marrow the virus dies after 12 days. This is a perfect 

 analogue to the experiment of Marchoux, 1 who cultivated the virus 

 of cyanolophia in a culture medium which contained red blood 

 corpuscles. He even believed that the living agents of cyanolo- 

 phia had multiplied and produced a much stronger virus than that 



1 Marchoux, "Cultures in vitro du virus de la peste aviaire," Compt. rend* 

 Acad. Sc., T. 147, p. 357, 1908. 



