Quantitative Determination of Plasma Protein. 197 



TABLE II. 



Results Averaged by Series. Effective Dilution of Serum Expressed in 



Decimals. 



Week. 



Heated 

 Control 



Heated . 

 Control 



Heated . 

 Control 



Heated . 

 Control 



Heated 

 Control 



.01250 

 .01050 



.00520 

 .00380 



.01500 

 .01000 



.00034 

 .00500 



.10000 

 .10000 



.00083 

 .00046 



.00078 

 .00023 



.00025 

 .00013 



.00017 

 .00514 



.01500 

 .01400 



.00020 

 .00008 



.00024 

 .00017 



.00017 

 .02600 



.01500 

 .01000 



.00010 

 .00008 



.00027 

 .00021 



.00010 

 .00008 



.01660 

 .01100 



In general our results confirm those of Graziani and suggest 

 that a moderately high atmospheric temperature (29°-32° C.) 

 tends slightly to decrease the power of agglutinin formation in the 

 rabbit. In Series IV alone this was not indicated. Here both 

 control rabbits gave abnormal results. No. 93 showed a marked 

 drop in agglutinating power during the third week; while No. 141 

 never formed any powerful agglutinins and died after the third 

 week. With the exception of this series there are sixteen weekly 

 averages of heated and control rabbits compared in Table II. In 

 these sixteen cases the effective dilution for heated and control 

 animals was on two occasions the same while in the other fourteen 

 instances a consistently larger amount of serum was needed to 

 produce agglutination in the case of the heated animals. 



112 (1176) 



Improved methods for the quantitative determination of plasma 



proteins. 



By Glenn E. Cullen and Donald D. Van Slyke. 



[From the Hospital of the Rockefeller Institute for Medical Research.] 



The blood is drawn into a tube containing an amount of po- 

 tassium oxalate sufficient to make 0.2 or 0.3 per cent, oxalate 

 solution, and is centrifuged twenty minutes. 



