2 



Scientific Proceedings (85). 



to the technique of Grigaut and of Autenrieth and Funk. Brown 

 colors do not develop as with the Bloor method. An aqueous 

 solution of naphthol green B, which is permanent, is used as 

 standard. 



Since the cholesterol esters give the color reaction, as pointed 

 out by Bloor, saponification is unnecessary. With this method 

 perfect duplicates and quantitative recoveries of added cholesterol 

 and cholesterol esters may be had. The results are considerably 

 lower than those obtained with the Bloor method. 



106 (1284) 



Morphin hyperglycemia as a test for pancreatic deficiency. 

 By John Auer and Israel S. Kleiner. 



[From the Department of Physiology and Pharmacology of the Rocke- 

 feller Institute.] 



We found that the subcutaneous injection of one or two 

 milligrams of morphin sulphate per kilo in dogs whose pancreatic 

 substance had been strongly reduced by coagulation in situ 1 or 

 by partial resection, caused a much greater rise in the blood-sugar 

 level than the same dose in normal controls. 



The following table gives the results of some of our experi- 

 ments. It will be seen that the animals in which the pancreatic 

 tissue had been reduced (AK5, 32, 37, and BD3) showed an 

 increase in the blood-sugar three to four times greater than that 

 obtained in the controls after the same dose of morphin. 



As these animals with deficient pancreatic tissue may legiti- 

 mately be considered in a prediabetic state, the morphin hyper- 

 glycemia observed in them may be of importance clinically in 

 detecting individuals with an impaired carbohydrate metabolism. 

 That this impairment need not be great and yet yield a strong 

 hyperglycemia to a small dose of morphin is indicated by the fact 

 that our dogs whose pancreatic tissue had been largely coagulated 

 nevertheless showed a surprisingly good tolerance for sugar. In 

 six tests where 10 grams of dextrose per kilo were fed, and in two 

 where 4 to 5 grams of dextrose per kilo were injected subcuta- 

 neously, the amount excreted was nothing in two tests; less than 



1 J. Auer and I. S. Kleiner, these Proceedings, 1917, XIV, 151. 



