SCIENTIFIC PROCEEDINGS 



Abstracts of Communications. 

 Eighty-seventh meeting. 



Rockefeller Institute for Medical Research, December ig, 1917. 

 President Gies in the chair. 

 125 (1303) 



The solvent action of antiseptics on necrotic tissue. 



By Herbert D. Taylor and J. Harold Austin. 



[From the Laboratories of the War Demonstration Hospital of the 

 Rockefeller Institute, New York.] 



The work which we wish to report was undertaken with the 

 idea of demonstrating the relative solvent action of the chlorinated 

 antiseptics on necrotic tissue, pus cell, erythrocytes, plasma clot, 

 and blood clot. 



Liver tissue was purposely infected, placed in the incubator 

 until thoroughly necrotic, shaken in salt solution with broken 

 glass until emulsified, strained through one layer of gauze, and 

 5 c.c. portions added to bottles containing 50 c.c. of the solutions 

 to be tested. 



After shaking at half-hour intervals for two hours, 15 c.c. 

 portions were removed and centrifuged for 5 minutes at the same 

 high speed in each instance. The volume of sediment thrown 

 down was measured, and by comparing the amount left after the 

 action of antiseptic solution with that remaining after the action 

 of control solutions of distilled water or salt solution, the amount 

 of solvent action could be readily determined. 



Dichloramine T is not soluble in water, and so oily solutions 

 had to be used either superimposed on an aqueous suspension of 

 the liver emulsion or alone. 



