44 



Scientific Proceedings (87). 



serum invariably offset the effects of aseptic inflammation, prob- 

 ably by neutralizing the virus as it passes through. Two separate 

 sets of experiments were carried out with Rosenow's serum, 

 normal horse serum, and immune monkey serum, in order to 

 determine whether the former contained neutralizing substances 

 rendering it suitable for therapeutic application. The results are 

 clearly decisive. 



Experiment I, Nov. 12: Macacus rhesus A (control) received 

 50 c.c. of clear supernatant fluid obtained by centrifuging a 5 per 

 cent, suspension of fresh active poliomyelitic tissue. The monkey 

 remained well. 



Macacus rhesus B. 5 p.m., November 11, received intra- 

 spinally 3.0 c.c. normal horse serum. November 12, 11:15 a.m., 

 received 50 c.c. of the same virus suspension as Monkey A received. 

 At 12 m. 3.0 c.c. normal horse serum were injected intraspinally. 

 Repeated daily intraspinal injections of 3 c.c. normal horse serum 

 were made until November 17, when the animal became slow. 

 Later in the same day the animal became prostrate and died 

 November 18. Typical lesions of poliomyelitis. 



Macacus rhesus C received at 5 p.m., November 11, 3.0 c.c. 

 activated Rosenow's horse serum intraspinally. On November 12 

 the intravenous injection of 50 c.c. of virus suspension was given, 

 followed by intraspinal injection of 3.0 c.c. activated Rosenow's 

 serum. The intraspinal injections were repeated daily. This 

 monkey became slow on November 17, excited on November 19. 

 On November 20 it dragged the right leg and climbed awkwardly. 

 November 23, legs were paralyzed and deltoids weak. November 

 24, prostrate. November 26 moribund; etherized. Typical 

 lesions of poliomyelitis. 



Macacus rhesus D received at 5 p.m., November 11, 3.0 c.c. 

 of pooled serum from monkey which had been paralyzed, re- 

 covered and reinforced by subcutaneous injections of active virus 

 suspensions. On November 12 Monkey D received an intravenous 

 injection of 50 c.c. of virus suspension, followed immediately by 

 an intraspinal injection of immune serum. The intraspinal in- 

 jections of the immune serum were continued for 6 days. The 

 monkey remained well. 



Experiment II differed from Experiment I only in the amount 



