Nutritive Factors in Animal Tissues. 



a half hours and there was no return of pain sense. In 8 dogs the 

 blood pressure sank to a low level but sensation of pain returned 

 when ether was discontinued. In the remaining 6 dogs sensation 

 was lost while the pressure was still above 95 mm. of mercury. 



In 10 other dogs etherization was pushed to the point of intoxi- 

 cation. Interpretation of the results of this series is not simple, 

 but it may be stated that the blood pressure, which fell in some 

 instances to 42 mm. often continued to rise for as much as two 

 hours after stopping the ether, eventually reaching a level above 

 90 mm. Pain sense sometimes returned after an even longer 

 interval, but eventually recovered more completely than the 

 blood pressure. 



151 (13^9) 



Further observations on the nutritive factors in animal tissues. 



By Lafayette B. Mendel and Thomas B. Osborne. 



[From the Sheffield Laboratory of Physiological Chemistry in Yale 

 University, and the Laboratory of the Connecticut Agricultural 

 Experiment Station, New Haven.] 



A recent communication 1 reported that the proteins of meat 

 (dried skeletal muscle) and of a glandular tissue, liver, are adequate 

 for the needs of nutrition in growth. The muscle contains a com- 

 paratively small quantity only of the water-soluble vitamine that 

 is an essential dietary factor, whereas liver tissue, on the other 

 hand, was found to be much richer in this. We have further 

 observed a similar adequacy of the proteins of heart, kidney and 

 brain tissue. The growth of rats to adult size has been secured 

 upon diets in which these tissues, used desiccated, furnished the 

 sole source of protein and water-soluble vitamine. In the cases 

 of the liver, kidney and heart, at least, fat-soluble vitamine also 

 seems to be present. The ether extract of pig's liver — liver oil — 

 has manifested growth-promoting properties comparable to those 

 described for butter fat and cod-liver oil; and it appears to be 

 efficient as a curative agent for the type of eye disease which we 



'Osborne, T. B., and Mendel. L. B., "Nutritive Factors in Animal Tissues," 

 I, Jour. Bio!. Chem., 1917, XXXII, 309. 



