9 2 



Scientific Proceedings (90). 



of blood taken or by a method described elsewhere, 1 i. e., by- 

 boiling the desired quantity of blood after the addition to each 

 c.c. of blood taken, of 5 c.c. of water, 1 drop of 10 per cent, acetic 

 acid and 2 drops of saturated solution of sodium acetate, cooling 

 and adding a few drops of 5 per cent, colloidal iron and water up 

 to 10 c.c. In either case after filtering, 1 c.c. of water-clear 

 filtrate equivalent to 0.1 c.c. of blood is treated in a micro-Kjeldahl. 

 After the reaction is completed, it is neutralized and water is 

 added up to 5 c.c. The color reaction can then be produced 

 directly in the tube after the addition of the reagents as follows: 



1. Add 1 c.c. of phenolate soda solution. (40 gm. phenol, 

 50 c.c. of 40 per cent. NaOH solution, water up to 100 c.c. mixed 

 in the cold.) 



2. Add 1 c.c. of 2 per cent, hypochlorite soda solution. Mix 

 well after addition of each reagent and add simultaneously to a 

 known standard or to each of a set of standards containing 5 c.c. 

 of water with 0.01 mgm., 0.02 mgm., 0.03 mgra., etc., of ammonia 

 nitrogen and place simultaneously with the unknown in boiling 

 water for five minutes. They are then allowed to cool, poured 

 into a graduate, made up to 10 c.c. with water and the unknown 

 compared with the known standard or standards. 



In case of ammonia the test is sensitive enough to detect and 

 estimate 1 part of ammonia nitrogen in a dilution of about 

 10,000,000. 



Details of the various methods will be published in the Journal 

 of Biological Chemistry. 



163 (1341) 



Asynchronism of the respiratory movements in lobar pneumonia. 



By Warren Coleman. 



[From the Department of Medicine, University and Bellevue Hospital 



Medical College.) 



Asynchronism of the respiratory movements occurs in many 

 severe cases of lobar pneumonia. The phenomenon was first 

 observed by the author some twenty years ago. A careful search 



1 Proc. of A. A. A. S., Dec, 1916, Theodore Kuttner, "A Modification of Folin's 

 Method for the Estimation of Creatinin in Blood." 



