2o (84) Society for Experimental Biology and Medicine. 



2 (48). "Preliminary communication on the composition of 

 the liver after subcutaneous injections of liver extracts " : 

 P. A. LEVENE and L. B. STOOKEY. 



About two years ago the authors observed that it was possible 

 to increase the resistance of rabbit blood against trypsin by treating 

 the animal to subcutaneous injections of pancreatic extract. It 

 seemed probable that the resistance of organs against other pro- 

 teolytic enzymes than trypsin could be increased in an analogous 

 manner. 



Rabbits were treated with saline liver extracts. The autolytic 

 powers of the livers of such animals were compared with the auto- 

 lytic powers of the livers of normal animals. It was found that 

 the autolytic power of the organ was not diminished by the treat- 

 ment referred to. It was also noted that the organs of the treated 

 animals contained smaller proportions of nitrogen than the livers 

 of the control animals, and further, that the proportions of nonco- 

 agulable proteins and of nonbasic nitrogen were higher than in the 

 controls. 



In order to accurately interpret the findings, the figures for 

 composition of the livers were compared. It was noted that the 

 amounts of water and carbohydrates were not affected by the 

 treatment, while the proportion of ether-extract was higher in the 

 organs of the treated animals than in those of the control animals. 

 The appended table gives the data of nine experiments, on as 

 many animals. 



Condition of 

 Rabbit. 



Weight of Rabbit. 

 Grams. 



Weight of Liver. 

 Grams. 



Gram Ethereal Ex- 

 tract per Gram 

 of Liver. 



Gram Carbohydrate 

 as Sugar per 

 Gram of Liver. 



Per Cent, of Water 

 in Liver. 



Nitrogen as c c. of 



- NH 4 OH per 

 10 



Gram of Liver. 



Liver Before Diges- 

 tion. 



Liver after Digest- 

 ing 24 Hours. 



Per Cent, of Total 

 Nitrogen as cc. of 



- W - NH 4 OH. 

 10 



Per Cent, of Total 

 Nitrogen asc c. of 



- NH 4 OH. 

 10 



Zinc Sul- 

 fate 

 Filtrate. 



Phospho- 

 tungstic 

 Filtrate. 



Zinc Sul- 

 fate 

 Filtrate. 



Phospho- 

 tungstic 

 Filtrate. 



Normal 













20.0 



13-5 



9-9 



18.O 



12.3 



< < 













18.O 



IO.O 



8.5 



24.O 



19.5 



t < 













I9.O 



10. I 



8.7 



18.9 



13.2 



< < 













I9.O 



12.6 



10.4 



18.9 



15-4 





2050 



75 



0.026 



O.078 



72.0 













«« 



2250 



87 



O.023 



O. 107 



69.2 



I9.8 





16.8 



28.5 





Treated 













16 O 



19-5 



22.5 



«« 



I7IO 



66 



O.O48 



O.055 



7-.5 



16.8 



21-5 



19.7 



30.7 



26.7 



< < 



2800 



76 



O.056 



O.071 



70.8 



17-3 



20.7 



15.6 



29.6 



23.2 



