22 Society for Experimental Biology and Medicine. 



carried out under the direction of Professor Schmiedeberg. Micro- 

 scopic examination of the tissues of rabbits killed by lethal doses 

 of this salt shows the deposition of metallic gold in and immedi- 

 ately about the nuclei of the cells of the kidney, liver, spleen, 

 mucosa of the gastrointestinal tract, and cardiac muscle. By 

 chemical analysis it was found that the greatest amounts of gold 

 were deposited in the kidney and liver, only traces having been 

 recovered from the other organs. 



On account of the ease of reduction of this salt, and the pos- 

 sibility of accurately estimating the metallic gold, it was recom- 

 mended as an indicator of the quantitative relationships of the 

 reducing processes in the various organs. 



19. " Effect of ligation upon the vital staining of nerves," 

 with demonstration : S. J. MELTZER. 



The author demonstrated pieces of dried sciatic nerves of 

 rabbits which, intra vitam, received intravenous infusions of meth- 

 ylene-blue. Single ligatures of the nerve, no matter where 

 applied, are without any influence upon the color of the nerve on 

 either side of the ligature. When the nerve is ligated at two 

 places the section between the two ligatures remains free of color, 

 while the central and peripheral ends of the nerve turn ;blue in 

 the usual manner. The effect is the same even if the ligatures 

 are applied near either end of the nerve. This fact shows that, 

 in the vital staining, the methylene-blue reaches the nerve only 

 from its central and peripheral ends. 



20. » Effects of bloodletting on metabolism " : PHILIP B. HAWK 

 and WILLIAM J. GlES. 



The author reported the results of some experiments re- 

 cently conducted by him in collaboration with Dr. Hawk. The 

 experiments were carried out on dogs in a state of nitrogenous 

 equilibrium. The withdrawals of blood were made while the 

 animals were under the influence of ether-chloroform. The 

 metabolic effects of anaesthesia and of operation were carefully 

 controlled. 



It was found that hemorrhages of about 3 Jo of body weight 

 caused, among other effects, (1) diminished secretion and de- 

 creased specific gravity, of the urine at first, the reverse in 



