Scientific Proceedings. 



37 



in the urine (after deducting the sugar fed in the cream), the rela- 

 tion between the two was found to be 3.65 gm. of dextrose to 

 I gm. of nitrogen, as follows : 



1904. 



Dextrose. 

 Grams. 



Nitrogen. 

 Grams. 



D : N. 







82.7 



23.O 



3.60 : I 







87.1 



23.8 



3-65 : I 







IOO.7 



27-5 



3.66 : I 



It will be noticed that the sugar and nitrogen rise and fall to- 

 gether. The amount of fat that was fed varied, but did not affect 

 the ratio. The sugar production is therefore parallel to the pro- 

 teid metabolism. Since 1 gm. of urinary nitrogen represents the 

 destruction of 6.25 gm. of proteid we can calculate the sugar pro- 

 duction from proteid. This D : N ratio is the same as that ob- 

 tained in our laboratory in phlorhizinized dogs. It has also been 

 obtained by others in the human subject, but has been falsely in- 

 terpreted as indicating the production of sugar from fat. It repre- 

 sents the maximum output of sugar from proteid and a complete 

 intolerance for carbohydrates. It is probably the most grievous 

 prognostic sign in diabetes. 



A calculation shows that the carbohydrates in the oatmeal and 

 levulose were nearly quantitatively eliminated in the urine when 

 the patient was under the influence of Diets I and II. 



The patient rapidly lost in weight and died in coma five weeks 

 after the completion of the above investigation. 



36. " Antihemolytic properties of the serum of nephrectomized 

 rabbits," with demonstration : S. J. MELTZER and WILLIAM 

 SALANT. 



In studying the properties of the blood of nephrectomized 

 rabbits it was found that bullock's serum, which is distinctly 

 hemolytic for normal rabbit's blood, was less so for the red cells 

 of nephrectomized rabbits. It was found, further, that the serum 

 of nephrectomized rabbits contains a distinct antihemolytic ele- 

 ment which is destroyed by heating for an hour at 58 0 C. On 

 the other hand the " washed" red cells of nephrectomized rabbit's 

 blood are at least no more resistant to the hemolytic influence of 

 bullock's serum than are the red cells of normal rabbit's blood. 



