So 



Scientific Proceedings (118). 



slight coagulating power for blood. The coagulating substance 

 which Mills isolated from various organs other than the pancreas 

 he identified as a protein-lipin. It might be added that thus far 

 we have not been able to demonstrate any lipin element in our 

 pancreatic rennet preparations. Therefore, it appears that our 

 rennet is not identical with the substance which Mills isolated. 



To determine the efficiency of pancreatic rennet as a blood 

 coagulant experiments were performed both in vitro and in vivo. 

 The test tube method of Lee and White 3 was used for estimating 

 the coagulation time. In the animal experiments, the individual 

 specimens were withdrawn from different vessels. 



We will first consider the effect of refined pancreatic rennet on 

 normal human blood. For this, blood was obtained from the 

 cubital vein of a normal individual in a series of small test tubes, 

 some containing CaCl 2 , others CaCl 2 and pancreatic rennet, and 

 still others containing rennet alone. The two controls which were 

 made, one at the beginning and one at the end of the experiment, 

 showed the coagulation time to be n and 12 minutes respectively. 

 The addition of CaCU to the blood reduced the coagulation time 

 to a period ranging between 5 and 8 minutes. Pancreatic rennet 

 alone reduced the coagulation time to 1 and i}4 minutes respec- 

 tively. Calcium chloride and pancreatic rennet, together, short- 



TABLE I. 

 Experiment i. 



a. Effect of CaCh i per cent, on the Coagulation of Whole Blood. 



Whole blood i c.c. i c.c. i c.c. 



CaCl 2 — 1 per cent I gtt. i gtt. 2 gtt. 



Norm, saline 1 gtt. 2 gtt. 3 gtt. 



Clotting time 8 min. 7 min. 5 min. 



b. Effect of Pancreatic Rennet on the Coagulation of Whole Blood. 



Whole blood 1 c.c. 1 c.c. 1 c.c. 1 c.c. 



Pan. rennet 1 gtt. 2 gtt. 3 gtt. 4 gtt. 



Saline 1 gtt. 1 gtt. 1 gtt. 1 gtt. 



Clotting time 1 min. 1 min. 1 min. 1^ min. 



c. Effect of CaCh and Pancreatic Rennet on the Coagulation of Whole Blood. 



Whole blood 1 c.c. 1 c.c. 1 c.c. 1 c.c. 



CaCh — 1 per cent 1 gtt. 1 gtt. 1 gtt. 1 gtt. 



Pan. rennet 1 gtt. 2 gtt. 3 gtt. 4 gtt. 



Clotting time 30 sec. 15 sec. 15 sec. 30 sec. 



•Lee. R. I., and White, P. D., A. J. Med. Sci., 1913, cxlv, 495. 



