82 



Scientific Proceedings (118). 



does not occur as is the case with saline lung extracts or other 

 tissue coagulants. 



TABLE III. 

 Experiment 3. 



Diluted citr. plasma 0.5 c.c. 0.5 c.c. 0.5 c.c. 



CaCl2 — 1 per cent 2 gtt. 2 gtt. o 



Pan. rennet — 1 per cent o 0 0.3 c.c. 



Clotting time 8 min. 7 min. 8 min. 



Experiment 4. 



Citrated 



plasma. .0.5 c.c. 0.5 c.c. 0.5 c.c. 0.5 c.c. 0.5 c.c. 0.5 c.c. 0.5 c.c. 0.5 c.c. 0.5 c.c. 0.5 c.c. 

 CaCh— 



1 per ct. . 2 gtt. 2 gtt. 2 gtt. 2 gtt. 2 gtt. 2 gtt. 2 gtt. 2 gtt. 2 gtt. 2 gtt. 



Rennet... 0 0.125 0.062 0.031 0.015 0075 0.038 0.014 0.007 0.003 



Saline. .. .0.25 — — — — — — — — — 



Clotting 



time. . . .6H m. 2 "m. 2 m. i}4 m. 2 m. z x A m. 4% m. 5 m. s l A m. 6 m. 



Cats were used in studying the effect of intravenous injection 

 of pancreatic rennet on blood coagulation. Moderate doses of 

 the rennet reduce the coagulation time, but not as strikingly as in 

 the experiments conducted in vitro. Excessive doses prolong the 

 coagulation, without giving rise to intravascular clotting. In this 

 respect also, the pancreatic rennet differs from the tissue coagu- 

 lants studied by Mills. The underlying mechanism of the short- 

 ening and prolongation of the clotting time thus produced is 

 being further investigated. 



The specific effects produced by the intravenous injection of 

 activated crude pancreatic extract, and the refined rennet, is 

 illustrated by the following experiments. 



Experiment 5. 



18 c.c. of a 3 per cent, activated crude pancreatic extract solution (rennet con- 

 tent 0.0054 g ) were injected into the jugular vein of a cat (under ether anesthesia). 

 The results obtained are shown in the following protocol. 



Clotting time before injection 7 minutes. 



Clotting time 5 minutes after injection 4 minutes. 



Clotting time 10 minutes after injection 2 minutes. 



Clotting time 20 minutes after injection 3 minutes. 



Clotting time 25 minutes after injection 3 minutes. 



Clotting time 40 minutes after injection 3 minutes. 



0.5 c.c. 

 2 gtt. 

 O.I c.c. 

 % min, 



0.5 c.c. 



2 gtt. 

 0.2 C.C. 



3^ min, 



0.5 c.c. 

 2 gtt. 

 0.3 c.c. 

 Hmin 



