9 2 



Scientific Proceedings (118). 



assumed that approximately half the glucose derived from protein 

 is used up in burning the ketogenic material from the a-amino 

 acids leucine, tyrosine and phenyl alanin occurring in the same 

 protein; no allowance is made in the expression for the possible 

 antiketogenic effect of the glycerol radicle present in the fats. 



Diets high in fat were fed to a normal subject, and to arthritic 

 patients undergoing the Pemberton 1 treatment. These diets were 

 based on that suggested by Shaffer 2 which contained 10 per cent, 

 of the total calories as protein, 10 per cent, as carbohydrate, and 

 80 per cent, as fat. The degree of acetonuria which corresponded 

 with each diet was determined, and the results compared with the 

 numerical values of this ratio. 



From a study of these values which corresponded with a very 

 mild degree of acetonuria it was concluded: one, that the phe- 

 nomenon of ketogenesis could properly be regarded as a molecular 

 reaction between ketogenic and antiketogenic compounds in the 

 diet; two, that protein entered into the reaction only to the extent 

 of the glucose which could be drived from the a-amino acids 

 contained in it; three, that the glycerol radicle of fat figured as a 

 source of antiketogenic material only to the extent to which glucose 

 could be derived from it; and, probably, four, that the glycerol 

 radicle probably did figure as a source of antiketogenic material 

 to the extent to which it could yield glucose. 



49 (1796) 



The glycogen content of the tissues of diabetic animals and the 

 influence of adrenalin thereon. 



By A. I. RINGER, H. DUBIN and F. HULTON FRANKEL. 



[From the Department of Physiological Chemistry, University of 

 Pennsylvania, Philadelphia, Penn.] 



In a series of experiments on dogs rendered diabetic by means 

 of phlorhizin, the glycogen content of the muscles was studied 

 immediately after the animals were killed. The muscles of thir- 

 teen animals were analyzed at the end of two days of glucosuria. 



1 Pemberton, R., Am. J. Med. Sci., 1917, cliii, 678. 



3 Shaffer, P. A., J. Biol. Chem., 1921, xlvii, 449; Woodyatt, R. T., Arch. Int. 

 Med., 1921, xviii, 125. 



