Glycogen in Tissues of Diabetic Animals. 



93 



The average glycogen content was 482 mgs. per one hundred grams 

 of muscle. The muscles of eleven animals at the end of the third 

 day of glucosuria contained an average of 306 mgs. per one hundred 

 grams. At the end of the fourth day, the muscles of twelve 

 animals contained an average of 228 mgs. of glycogen per one 

 hundred grams. At the end of the fifth day seven animals showed 

 an average of 155 mgs. per one hundred grams of muscle, and at 

 the end of the seventh day two animals showed the presence of 

 124 and 151 mgs. per one hundred grams of muscle. 

 The detailed results are summarized in Table I. 



table 1. 



Glycogen Content of Muscle of Diabetic Dogs. 

 Days of Glucosuria. 





2 



3 



4 



5 



7 





723 



250 



181 



in 



124 





626 



38i 



237 



097 



151 





573 



337 



226 



080 







415 



499 



200 



315 







430 



236 



144 



293 







325 



305 



130 



no 







295 



121 



120 



105 







447 



192 



297 









435 



356 



282 









528 



378 



3i9 









628 



306 



316 









407 





286 









440 











Average .... 



482 



306 



228 



155 



138 



Throughout these periods, the animals fasted and were given 

 phlorhizin injections by the Coolen method (one gram daily in 

 olive oil). 



These findings prove that in spite of continuous fasting and in 

 spite of complete diabetes, the muscle cells will hold on to a certain 

 amount of glycogen, which we may term residual glycogen. 



In a second series of experiments, diabetic phlorhizinized dogs 

 were given injections of three to seven milligrams of adrenalin, and 

 were killed twenty-four hours after that. The injections were 

 made on the second or third day of glucosuria. 



The analysis of the muscles of five dogs of this series showed 

 that absolutely no glycogen was present in them, proving that 



