n8 Scientific Proceedings (119). 



Attempts to produce contraction by nervous stimulation were 

 unsuccessful. Strong direct faradic stimulation of the organ 

 failed to produce contraction or expulsion of contents. 



A series of experiments, in which phenoltetrachlorphthalein was 

 injected intravenously and subsequently recovered when excreted 

 in the bile, showed the appearance of this substance in the duode- 

 num in from ten to fifteen minutes after injection. A flow into the 

 gall bladder of liver bile was demonstrated by the recovery of the 

 phenoltetrachlorphthalein from the bladder bile when the cystic 

 duct was patent. None of the dye was found in the sac when the 

 duct was tied off. 



Observations on the filling of gall bladders emptied by digital 

 compression showed the filling to be a slow and irregular process 

 in spite of the fact that the bile flow into the duodenum may or 

 may not be continuous. Bile flow into the duodenum was ob- 

 served with regularity in the cases of fasting dogs as also in the 

 absence of immediate digestion. 



Stasis of gall bladder contents was investigated in a series of 

 experiments in which, under aseptic precautions, a sterilized 

 suspension of an inert finely divided substance (charcoal or 

 carmine) was introduced into the gall bladder. The dogs were 

 permitted to live for periods of 12 hours, I, 3, 7 days. During 

 these periods they were fed a mixed ration of protein, fat, car- 

 bohydrate and water. At autopsy, the coloring matter was 

 recovered from the gall bladders up to and including three days. 

 No traces were found in intestinal washings. The bladder was 

 found to have emptied in one week. Patency of the ducts was 

 shown by the slight digital pressure needed to expel the bladder 

 contents. 



