Inorganic Phosphate of Blood. 171 



the plasma. This seems not to be the case in the blood after it is 

 taken from the body. 



TABLE 11. 



To Illustrate Effect of Time after Drawing Blood on the Inorganic 



Phosphate. 





Within y 2 Hr. 



Within 1 Hr. 



After 5 Hrs. 



After 20 Hrs. 



I. 







2.4 



2-5 







Blood 



2-5 



2.8 



3-3 





II. 







2-55 





2-53 





Blood 



2.6 



3-08 





4.02 



These results lead to the following conception of the role of 

 inorganic and organic acid soluble phosphorus in the blood: The 

 red cell is totally permeable to the phosphate ion, i.e., no "osmotic 

 influences" control the distribution of the phosphate ion inside 

 and outside of the cell. Phosphate ions can be taken up by the 

 cell and stored as organic acid soluble phosphate (Iverson). This 

 organic phosphate is hydrolyzed very easily when there is need 

 for phosphate ion in the plasma, similar to the liver glycogen 

 yielding blood sugar. The diffusion of the phosphate out of the 

 cell, however (at least in vitro), is slower than its rate of forma- 

 tion by hydrolysis. To substantiate this view we will still have 

 to show under what conditions inorganic phosphate can diffuse 

 out of the cell. We have no indication so far that phosphate 

 distribution is influenced by the CO2 tension. We are now col- 

 lecting data on this point, as well as on the whole subject from 

 the point of view of the acid soluble phosphate. 



79 (1826) 



Observations on the inorganic phosphate of blood in experimental 



rickets of rats. 



By M. B. GUTMAN and V. KNEELAND FRANZ (by invitation). 



[From the Department of Pathology, College oj Physicians and 

 Surgeons, Columbia University, New York City.] 



The work of Howland and Kramer 1 on the level of the inor- 

 ganic phosphate in the blood in human rickets, and some con- 



1 Howland, J., and Kramer, B., Amer. Jour. Dis. of Child., 1921, xxii, 105. 



